2nd Grade Essay Topics

2nd Grade Essay TopicsThere are many 2nd grade essay topics for you to choose from. You can use this information to help determine the topics that will work best for your child. Make sure that you choose topics that will be educational and enjoyable for your child. Essay topics should stimulate your child's mind as well as giving him a sense of accomplishment.When preparing for the next school year, you will want to take some time to learn about the subjects that will be on the test. You can find out about the subjects by talking to your child's teacher. It will also be helpful to make a list of all of the subjects that you believe your child will need to know for his/her 2nd grade assignment.If you do not want to spend a lot of time researching the subjects for your child's 2nd grade homework, then you may want to consider purchasing a student guide to help you with the assignments. The student guide will provide you with outlines for the many different subjects that your child will need to know. Some of the topics that your child will need to know include American History, Science, Geography, Math, Government, History, and English. Some of the other topics will be about Drama, Writing, Music, and Math.Two of the best 2nd grade essay topics are about 'American Idol'Colonel Sanders.' Both of these topics are interesting to your child. When he hears about a particular celebrity, he may get excited about how the person is doing and want to visit that location.A topic about American History may be one that your child is interested in. After all, it has been a very long time since our country was able to boast a strong national identity. You can use your child's personal experiences to share his/her own thoughts on the subject.A topic about Science may also be something that your child would be interested in. If you are in the teaching profession, you can also use this information to bring science into your lesson plans. Science can be an exciting subject to discus s with your child.A topic about Geography is a topic that can provide your child with a background for General History. Your child will learn about various historical places that have shaped our country. As he reads the information in the assignment, he will find out what it means.It is up to you as to which subjects your child will need to read about for his/her 2nd grade year. There are many topics that you can use as a base to create your own topics. However, when you first start out, it may be helpful to begin with the most popular topics to give yourself a base to work from.

Signal Transduction Exam 2018 Free Essays

This figure is from Labasque et al., 2008 (GPCR week). A) Describe the experiment shown here and what was learned as a result. We will write a custom essay sample on Signal Transduction Exam 2018 or any similar topic only for you Order Now B) Design a negative control for this experiment and describe why it is a good control. C) Design an experiment to confirm this result in living cells. Please include controls in your experimental design. Signal Transduction Exam 2018 A) The experiment was performed on human embryonic kidney (HEK 293) cell line. The extracts of solubilized proteins were taken under consideration for this experiment. These were then transfected with different vectors: CTL (empty vectors), co-transfected with GFP fused Calmodulin(GFP-CaM) and serotonin receptor either wild type 5-HT2C or 5-HT2CR376/377A which were treated with a vehicle or 5-HT for five minutes, they analyzed the binding reaction by Western Blot and Immunoprecipitation (IP) techniques. Immunoprecipitation was done of these soluble proteins with polyclonal antibody:anti-GFP. Furthermore, these immunoprecipitated proteins were blotted using a monoclonal anti-GFP antibody along with anti-5-HT2C receptor antibody.As a result of this experiment, it was found that the absence of agonist, 5-HT2C receptor did not co-immunoprecipitate with calmodulin in solubilized proteins that were co-transfected with Myc-5-HT2C receptor and GFP-CaM. However, serotonin receptor co-immunoprecipitated with GFP-CaM after treatment with 5-HT for 5 minutes. But the exposure with 5-HT2CR376/377A did not co-immunoprecipitate GFP-CaM with serotonin receptor. It proves that 5-HT2C receptors are linked with CaM depending on the presence of agonist. The experiment performed is to check the agonist dependent precipitation of GFP-CaM and serotonin receptors in the presence of Myc-5HT2C 5-HT exposure. So, the negative control to be designed here for this experiment can be simply of serotonin receptor and CaM. This would result in no immunoprecipitation and prove as a good negative control. On analyzing it through western blotting it could be proved that no immunoprecipitation had occurred in this control. To confirm the results in living cells the same experiment could be used to check the that whether the immunoprecipitation is agonist dependent or not. Take solubilized proteins from living cells and co-transfect them with GFP-CaM and 5-HT2C receptor followed by exposure to 5-HT, then analyze immunoprecipitation via western blotting. The negative control for this experiment will include only CaM and serotonin receptor, and further analyzing it with western blotting, the results of blotting will show no precipitation in negative.2. These figures are from Fig. 6A,C of Fan et al. (src kinase folder). (A) Explain the experiments shown here. What was being tested? What conclusions can be drawn? How can it be improved? (B) Design another experiment to test the same concept/hypothesis? Include your controls.4839335107886500A) In figure A: Human embryonic kidney cells were transfected with constructs for carrying out this experiment. Immuno-precipitation was carried out of tagged PAG with an antibody against regulator MYC, its interaction is studied here with SRC and BRK followed by immunoblotting analyzation. The result of this experiment was delaying in electrophoretic mobility of tagged PAG protein, when it was co-expressed with SRC. This delay is considered due to the hyper-phosphorylation. However, on co-expression of PAG and BRK this band shift was less evident. In figure C: Co-transfection of HEK 293 cells with MYC-PAG and SRC followed by treatment in the absence or presence of SRC kinase inhibitor SU6656 (5 M) for at least 1 hour. PAG was immunoprecipitated by antibody against MYC, and the binding of CSK was compared by immunoblotting. On the other hand, the disturbance of link b/w CSK and PAG was observed on weakening SRC activity by a small molecular inhibitor SU6656, this experiment concluded the importance of SRC kinase activity for functioning of CSK. Also, this study shows that distinct mechanisms have evolved to regulate the activities of two structurally similar and functionally related kinases, BRK and SRC, understanding of the signaling function of protein phosphatases, including definition of their substrate specificities, will allow us to exploit a greater spectrum of the changes in signaling in disease and to generate new and more effective strategies for therapeutic intervention in major human diseasesB) To test the same hypothesis with controls, take breast cancer frozen samples of different patients, divide the sample in two groups one will be the test group other the control group. The control group will be given doses of anti-tumor medication (like tamoxifen for breast cancer). RNA will be extracted from both groups using Trizol and will be followed by purification assay. The breast cancer cell lines will be transfected with empty vectors or pcDNA3-MKP3-V5. Furthermore, the transfectants will be placed in media like minimum essential media MEM with phenol red, followed by SDS PAGE electrophoresis, and transfer to nitrocellulose membrane. After transferring they will be incubated with primary antibody for an hour or more then with the secondary antibody to observe the chemiluminescence with a reagent. The cells will then be lysed in a buffer, phosphatase reaction carried out will be observed by assays and transfected cells will be compared with control ones to whom tamoxifen was given.36734750003. This is figure 4b from Gresset et al (phospholipases folder). (A) What is the hypothesis being tested in this experiment? (B) Describe how the experiment was done, including results. (C) Predict what might happen to PLC? activity if Tyrosine783 was ‘permanently’ phosphorylated and explain why. The hypothesis being tested here is the enhancement in the lipase activity of phospholipaseC-?1 via phosphorylation of one or both specified tyrosine residues (Y775 and Y783) in the downstream process of signal transduction.To perform the experiment, equal concentrations of purified phospholipase-C-?1 were set on incubation with the active kinase domain of the Fibroblast growth factor receptor FGFR2 and ATP in bovine serum albumin containing buffer, the samples of this reactions were tested for two activities: 1) for lipase activity in the phospholipid vehicles indicated in the figure on left Y axis. Secondly the phosphate incorporation in phospholipase-C-?1 was studied, illustrated at right Y axis of the figure. 4 moles of phosphate were added to each test samples, the Wildtype inactive PLC?1 was taken as a normal control with its basal phospholipase activity, active PLC?1 with wildtype FGFR2K served as a positive control with phosphate and PLC?1 knockout mutant served as negative control to quantify the phospholipase activity among the mutants. This was performed to check the phosphorylation of tyrosine and auto inhibition of PLC-? isozymes, 775/783 of PLC-?1 were substitutes at the place of phenylalanine, they could be used individually or together, but in the experiment tyr783 is used individually. Phospholipase activity of resulting mutant after purification was quantified with active domain of FGFR2K (helps in phosphorylation and activation of phospholipase). Certain known moles of phosphates were added into purified PLC-?1 in wild type under above mentioned conditions and observed that phospholipase activity was enhanced 10 times. The mutation of tyr783 completely nullified the kinase stimulated acceleration of phospholipase activity along with reduction in FGFR2K-promoted phosphorylation of PLC-?1. This experiment proves that, phosphorylation of Tyr783 is vital for relief of auto-inhibition.Studies reveal that Tyr-783 was essential for auto inhibition. As discussed above, permanent phosphorylation of tyr-783 will completely nullify the kinase stimulated and FGFR2K stimulated phosphorylation of PLC-?1. Lipase activity of PLC-?1 will be enhanced across its limits and over-expression of PLC-?1 can induce malignant transformation. The results could be leading to production of carcinoma cells. It has been found in various studies that activity of PLC-?1 is more in cancerous cells as compared to normal cells. So, permanent phosphorylation tyr783 could be a way leading to malignant cancers.3416300-254000004. This figure is from Tsui et al. 2015. (lipid raft folder). (A) Explain the relationship between GFR and Ret51 and what they are testing in this paper (the overall idea). (B) Explain the experiment shown in this figure and what was learned. (C) What is a negative control that could improve the conclusions from this experiment?GFR and Ret51 both are receptors, GDNF is found to promote PNS development and kidney morphogenesis through the receptor complex consisting of GDNF family receptor 1 (GFR1) and the other receptor tyrosine kinase (Ret). Ret signal transduction is increased by translocation of GFR. GFR-mediated Ret activation is essential too for the kidney morphogenesis and for various other functions of abdominal precursors that form abdominal nervous system. Also, GFR has many lipid rafts because its GPI anchorage, but Ret is expelled from lipid rafts. In this paper, the gene replacement for GFR in mice results GDNF resulting in Ret activation but prevented its translocation into lipid rafts. These mice showed renal agenesis, and other disorders including loss of the enteric nervous system, and defects in motor neuron axon path similar to GFR mice that was knocked out, all this provided evidence along with lipid rafts GFR is also needed for neurotrophic factor signaling.Primary considerate neurons secluded from Gfr1and Gfr1TM/TM mice were maintained in vitro for some days. Then they treated the neurons with GDNF or medium for exact time of 15 minutes. The Detergent-resistant membranes quarantined from the neurons were examined by immunoblotting for Ret51. The comparative purity of detergent resistant and detergent soluble fractions was confirmed by using immunoblotting for caveolin and transferrin receptor, respectively B, the experiments shown in A, were computed and graphed. Otherwise, Substantial decline in the amount of Ret51 was recorded statistically that translocated into lipid rafts while GDNF stimulation in Gfr1TM/TM neurons compared with Gfr1 neurons. Similar Results were obtained after performing the experiment four times .Moreover, Lipid raft translocation experiments were performed to prove the concept that GDNF/GFR1/Ret complex does not translocate into lipid rafts in Gfr1TM/TM mice. Primary sympathetic neurons from Gfr1/ and Gfr1TM/TM mice were used to extract detergent-resistant membranes. Upon stimulation of Gfr1/ neurons with GDNF, Ret translocated quickly into lipid rafts. This was a contrast to Gfr1TM/TM neurons that an evident reduced movement of Ret into the detergent-resistant was recorded because of GDNF exposure. A small portion of Ret that did translocate into lipid rafts while stimulation may be owing to Ret kinase-dependent translocation of Ret into rafts that occurs with slower movements. There was a significant, 75% reduction in the kinetics of the Ret receptor complex into lipid rafts during GDNF exposure in Gfr1TM/TM neurons according to computation made by these experiments. The negative control design here for confirming the results that Ret doesn’t translocate into lipid rafts during GFL activation in Gfr1TM/TM neurons, the primary sympathetic neurons isolated from Gfr1/and Gfr1TM/TM mice will be grown in the same way as test ones, with the only difference that there will be no treatment with GDNF or medium for 15 minutes, and the impact of this will confirm the result to much greater extent upon immunoblotting. How to cite Signal Transduction Exam 2018, Papers

Of Mice And Men Appreciation Essays - English-language Films

Of Mice And Men Appreciation The book that I have read that has really stayed with me is Of Mice and Men by John Steinbeck. I really enjoyed reading it which is unusual because I usually don't enjoy reading too much. There was something about George and Lennie's friendship that really made me think. Seeing how they were and how they shared life was really interesting. George didn't have to bother with Lennie, he could have abandoned him and gone on his own way. But he did not do that, he stayed with Lennie watching over him almost like a parent to a child. Even though Lennie always got George in trouble, George never stopped loving him and always stood by him. The friendship they shared went beyond what was transparent they each shared a dream and both knew they meant the world to each other. I felt that if these totally different people could get along and look out for each other, why cannot we get along with people who are different than us. They made me realize that I could learn something from how to treat people who are different than me. What I also liked about it was the way they never stopped trying to reach their dream. This made me think that if they could work hard for there dream why can't I. It showed me that it does not matter were you come from or what you do, it is okay to dream and work as hard as you can to reach it. For all it shows for friendship and loyalty it also shows how sometimes you have to do things you never thought you would do. For example in the end when George is forced to shoot Lennie in the head you would never have thought he would do that, but you can see that under the circumstances he had no other choice. He only had two choices let the other people get to him first and watch them torture Lennie while he died a long horrible death or do it himself and get it over quick were Lennie did not know what hit him. This is also true in life, many times we are faced with tuff choices and even though they may be the hardest you will have to go through, you know that that is the only way. You come to the realization that everything you thought you was about, can all change with a blink of the eye.

The Upside to Online Homework Help

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Research Paper Topics on Historical Events

Research Paper Topics on Historical EventsWriting your research paper topics for historical events can be difficult, but with the correct research method you will find that you will not only get great ideas, but your paper will also come out great. The most important thing to remember is that there are no rules as to what historical event can be used, nor do you have to use any. However, you do need to remember that historical events are still historical events, so if you use an event that was a little inaccurate you will ruin your credibility and you will be seen as a fraud.The best way to research paper topics on historical events is to get some help from a professional, and if you don't want to do that then there are plenty of books available that will provide you with the information you need. I would suggest you get one that has some expertise in the field you are studying, because it will make your life a lot easier when it comes to writing your paper. If you cannot get any of these books locally, you will probably be better off using the internet to help you with your research.Remember that writing about historical events and getting help with research paper topics is not a big deal; you will be surprised what you learn about history by doing this. You do not have to be an expert to write these papers. You should not feel that you need to be an expert to write history, and once you have written the first article on your topic you will feel like you have made a start. Try not to stop at articles, as these are the key to getting more experience with writing about your topic.The key to research paper topics on historical events is to keep on reading more about the subject you want to write about. For instance, if you are writing about an old battle, you might want to find a book on the battle or newspaper archives to find the historical facts. There are plenty of books available on just about every subject you can think of, so it won't be hard to find enoug h information to write about. If you feel you are lacking the information you need, then simply ask someone you know for some advice.When you are writing your research paper topics on historical events you need to remember that many of the books on your topic are quite detailed and that you should take time to read them. You need to understand every topic you are researching before you start writing. You may find a single detail or snippet of information is enough to spark an idea and you may want to follow this up with another article.Another thing to keep in mind when researching historical events is that many of these articles will become obsolete before you finish your course. This means that if you write two or three articles then you will have to write a research paper on each one. While you can never tell when you will need to write your research paper on a certain topic, you should do the best you can with your current knowledge.There are plenty of books available on histori cal events, but you should always go for the ones that were written by a professional. This will give you a solid base to work with and will help you with future research papers. There are many e-books available which can also be useful, but again, you should always try to get the best information from a book written by a professional.Hopefully this article has helped you get the best information on research paper topics on historical events. The key to getting the perfect topic is just a matter of sticking to a few simple rules, and you will soon find that you have an article that is completely original.

The Downside Risk of College English Essay Writing Service Review That No One Is Talking About

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A World History Sample Long Essay - Makes Your Essay Very Interesting

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